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《PNAS》:我科學家為超級雜交稻產量相關位點的解析提供了理想平臺 
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發布時間:2013-08-14

8月12日,《PNAS》雜志在線發表了由中國水稻研究所、深圳華大基因研究院和中國科學院遺傳與發育研究所等單位合作的最新科研成果。這篇題為“Dissecting yield-associated loci in super hybrid rice by resequencing recombinant inbred lines and improving parental genome sequences”的論文,為超級雜交稻產量相關位點的解析提供了寶貴的材料和有效的途徑。

中國的雜交稻為世界糧食生產作出了巨大貢獻,作為超級雜交稻標桿組合的兩優培九以其豐產特性,在生產上大面積種植。研究人員通過長期努力,構建出132個超級雜交稻兩優培九的核心重組自交系和1709個用于基因克隆的大規模重組自交系,并進一步對核心重組自交系進行了基因組重測序,成功構建了超高分辨率的遺傳連鎖圖譜。

基于高密度物理圖譜,研究人員不僅更新了兩優培九的父本品種93-11和新組裝的母本品種培矮64s的基因組序列,還檢測到43個與水稻產量密切相關的數量性狀位點(QTL),其中包括20個尚未報道的新QTL。同時,利用大規模重組自交系群體,結合親本基因組間大量精確的單核苷酸多態標記和插入缺失標記對兩個QTL——qSN8和qSPB1加以精細定位,分別確定DTH8和LAX1為候選基因,其中qSN8通過互補實驗證實為DTH8基因。該項研究還為進一步闡明超級稻產量的遺傳基礎以及分子育種應用搭建了理想平臺。

論文摘要:

The growing world population and shrinkage of arable land demand yield improvement of rice, one of the most important staple crops. To elucidate the genetic basis of yield and uncover its associated loci in rice, we resequenced the core recombinant inbred lines of Liang-You-Pei-Jiu, the widely cultivated super hybrid rice, and constructed a high-resolution linkage map. We detected 43 yield-associated quantitative trait loci, of which 20 are unique. Based on the high-density physical map, the genome sequences of paternal variety 93-11 and maternal cultivar PA64s of Liang-You-Pei-Jiu were significantly improved. The large recombinant inbred line population combined with plentiful high-quality single nucleotide polymorphisms and insertions/deletions between parental genomes allowed us to fine-map two quantitative trait loci, qSN8 and qSPB1, and to identify days to heading8 and lax panicle1 as candidate genes, respectively. The quantitative trait locus qSN8 was further confirmed to be days to heading8 by a complementation test. Our study provided an ideal platform for molecular breeding by targeting and dissecting yield-associated loci in rice.

詳細信息:

http://www.cnrri.cn/zjww/Detail.aspx?id=20024329

http://www.pnas.org/content/early/2013/08/08/1306579110.abstract

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