首頁 > 科研前沿  > 《Plant Physiology》:發現水稻受體類激酶OsSIK2提高耐鹽性并延緩衰老  
《Plant Physiology》:發現水稻受體類激酶OsSIK2提高耐鹽性并延緩衰老 
作者:中國水稻信息網
來源:中國水稻信息網
發布時間:2013-10-25

10月18日,Plant Physiology在線發表了由我國科學家研究發現的最新研究成果。受體類激酶在植物生長發育和防御反應中起著非常重要的作用。它們在非生物脅迫反應中的作用研究較少。

在這篇題為“An S-domain receptor-like kinase OsSIK2 confers abiotic stress tolerance and delays dark-induced leaf senescence in rice”(doi:10.1104/pp.113.224881)的論文中,來自中科院遺傳所的研究人員研究發現,水稻的一個具有S-結構域的受體類激酶OsSIK2參與調控非生物脅迫反應和衰老過程。研究結果表明,對于OsSIK2 的改造和應用可能有利于水稻和其它作物的改良和穩產。

OsSIK2定位于細胞質膜,在Mn2+存在下具有激酶活性。OsSIK2基因主要在水稻葉片和葉鞘表達,其表達受鹽、干旱、冷害和植物激素ABA的誘導。過表達OsSIK2的水稻株系同對照株系相比呈現對鹽脅迫和干旱脅迫的耐性。而突變體在這些脅迫處理后出現更加敏感的癥狀。當把OsSIK2的胞外域刪除之后,表達截短蛋白的株系呈現比表達全長蛋白的株系更高的耐鹽性。上述功能的差異可能是通過激活不同的下游基因引起的。全長蛋白可增強PR類基因的表達,而截短蛋白可以促進DREB類基因的表達。OsSIK2過表達株系還出現葉片發育早及黑暗誘導的衰老延遲的表型。全長蛋白和截短蛋白激活的下游基因均受鹽脅迫、干旱脅迫和黑暗處理的誘導。這些結果表明,OsSIK2可能整合脅迫信號于發育過程從而使植物在不利環境條件下進行適應性生長。

根據2013年ISI發布的JCR(Journal Citation Reports)數據,Plant Physiology最新SCI影響因子為6.555,在總計197種SCI期刊的植物學科(PLANT SCIENCES)中排名第8位。在過去的12年間,該刊物影響因子處于5.105至6.555之間,并呈逐年穩步上升趨勢。


 

論文摘要:

Receptor-like kinases (RLKs) play important roles in plant development and defense responses; however, their functions in other processes remain unclear. Here, we report that OsSIK2, an S-domain RLK from rice, is involved in abiotic stress and senescence process. OsSIK2 is a plasma membrane-localized protein with kinase activity in the present of Mn2+. OsSIK2 is expressed mainly in rice leaf and sheath, and can be induced by NaCl, drought, cold, dark and ABA treatment. Transgenic plants overexpressing OsSIK2 and mutant sik2 exhibit enhanced and reduced tolerance to salt and drought stress respectively compared to the controls. Interestingly, a truncated version of OsSIK2 without most of extracellular region confers higher salt tolerance than the full-length OsSIK2, likely through activation of different sets of downstream genes. Moreover, seedlings of OsSIK2-overexpressing transgenic plants exhibit early leaf development and delayed dark-induced senescence phenotype, while mutant sik2 shows opposite phenotype. The downstream PR-related genes specifically up-regulated by full-length OsSIK2 or the DREB-like genes solely enhanced by truncated OsSIK2 are all induced by salt and dark treatments. These results indicate that OsSIK2 may integrate stress signals into developmental program for better adaptive growth under unfavorable conditions. Manipulation of OsSIK2 should facilitate improvement of production in rice and other crops.

詳細信息:

http://www.cnrri.cn/zjww/Detail.aspx?id=20024533

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上篇文章:《The Plant Cell》: 水稻理想株型研究獲新進展
下篇文章:《PNAS》:我科學家發現新型水稻抗旱基因
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